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elisa plate blocking buffer  (Elabscience Biotechnology)


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    Elabscience Biotechnology elisa plate blocking buffer
    Elisa Plate Blocking Buffer, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/elisa plate blocking buffer/product/Elabscience Biotechnology
    Average 94 stars, based on 6 article reviews
    elisa plate blocking buffer - by Bioz Stars, 2026-06
    94/100 stars

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    mice vaccination and antibody induction. (A) mice groups. Mice were divided into 4 groups with 15 mice in each group. Mice vaccinated with DPBS were used as negative control. (B) mice vaccination and samples collection. LNP-mRNA was administered intramuscularly at day 0 and 14. Blood was collected at day 7, 13, and 35. One group of mice was sacrificed at day 30 to collect the spleens. Mice were intranasally challenged with a lethal dose (1×10 6 pfu) of WR vaccinia virus. (C and D) A35R <t>and</t> <t>M1R</t> specific total IgGs, respectively. Serum with different dilutions at different timepoints was used for <t>ELISA</t> against A35R or M1R. (E) Neutralizing antibodies were measured. The sera were diluted and incubated with the virus for PRNT. Serum from DPBS group were regarded as 0% neutralization.
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    mice vaccination and antibody induction. (A) mice groups. Mice were divided into 4 groups with 15 mice in each group. Mice vaccinated with DPBS were used as negative control. (B) mice vaccination and samples collection. LNP-mRNA was administered intramuscularly at day 0 and 14. Blood was collected at day 7, 13, and 35. One group of mice was sacrificed at day 30 to collect the spleens. Mice were intranasally challenged with a lethal dose (1×10 6 pfu) of WR vaccinia virus. (C and D) A35R and M1R specific total IgGs, respectively. Serum with different dilutions at different timepoints was used for ELISA against A35R or M1R. (E) Neutralizing antibodies were measured. The sera were diluted and incubated with the virus for PRNT. Serum from DPBS group were regarded as 0% neutralization.

    Journal: bioRxiv

    Article Title: Novel mRNA vaccines encoding Monkeypox virus M1R and A35R protect mice from a lethal virus challenge

    doi: 10.1101/2022.11.19.517190

    Figure Lengend Snippet: mice vaccination and antibody induction. (A) mice groups. Mice were divided into 4 groups with 15 mice in each group. Mice vaccinated with DPBS were used as negative control. (B) mice vaccination and samples collection. LNP-mRNA was administered intramuscularly at day 0 and 14. Blood was collected at day 7, 13, and 35. One group of mice was sacrificed at day 30 to collect the spleens. Mice were intranasally challenged with a lethal dose (1×10 6 pfu) of WR vaccinia virus. (C and D) A35R and M1R specific total IgGs, respectively. Serum with different dilutions at different timepoints was used for ELISA against A35R or M1R. (E) Neutralizing antibodies were measured. The sera were diluted and incubated with the virus for PRNT. Serum from DPBS group were regarded as 0% neutralization.

    Article Snippet: A35R protein (OkayBio, C1620) and M1R protein (OkayBio, C1624) were diluted to 5 μg/ ml by ELISA coating buffer (Elabscience, E-ELIR-003) and added into 96-well plates for overnight coating at 4 degree.

    Techniques: Negative Control, Virus, Enzyme-linked Immunosorbent Assay, Incubation, Neutralization